Imaging Flow Cytometry: Methods And Protocols Here

While IFC shares many preparatory steps with conventional flow cytometry, it requires specific optimizations to ensure high-quality imagery.

: Cells must be in a single-cell suspension to avoid clogging the thin fluidic channels used for imaging. Imaging Flow Cytometry: Methods and Protocols

: Beyond fluorescence, IFC provides data on cell size, shape, circularity, and internal complexity. While IFC shares many preparatory steps with conventional

: If internal markers (e.g., nuclear proteins) are the target, cells are fixed with formaldehyde and permeabilized with detergents like Triton X-100. : If internal markers (e

: Non-specific binding is prevented using anti-Fc antibodies. Cells are then stained with fluorophore-conjugated antibodies, viability dyes, or nuclear dyes like DAPI or Hoechst to provide spatial landmarks.

: Researchers must set specific software parameters, such as magnification and laser power, to maximize the resolution of the captured images. Common Applications