Electrophoresis

Electrophoresis Link

An electrical current is applied. Since DNA and RNA are negatively charged due to their phosphate backbone, they migrate toward the positive electrode (anode).

Small molecules move through the pores of the gel quickly, while larger molecules get tangled in the matrix and move more slowly. Over time, the molecules separate into distinct bands based on their molecular weight. Common Types Electrophoresis

Used for proteins. The detergent SDS unfolds the proteins and gives them a uniform negative charge, ensuring they are separated strictly by length rather than shape. An electrical current is applied

DNA profiling (DNA fingerprinting) uses electrophoresis to compare crime scene samples with suspect DNA. Electrophoresis

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